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D. Richter (auth.), Hiroo Imura M. D. (eds.)'s Recombinant DNA Technologies in Neuroendocrinology PDF

Posted On April 21, 2018 at 12:34 am by / Comments Off on D. Richter (auth.), Hiroo Imura M. D. (eds.)'s Recombinant DNA Technologies in Neuroendocrinology PDF

By D. Richter (auth.), Hiroo Imura M. D. (eds.)

ISBN-10: 3642775039

ISBN-13: 9783642775031

ISBN-10: 3642775055

ISBN-13: 9783642775055

This quantity covers new facets and destiny instructions in molecular neuroendocrinology, an incredible and speedily becoming sector in neuroendocrinology. one of the a number of neurotransmitters or neuromodulators that play a massive function within the keep watch over of endocrine features, neuropeptides and similar proteins have drawn certain cognizance due to their variety and complexity in motion. extra lately, molecular biology has turn into a necessary software of study during this sector. a number of genes encoding neuropeptides and different similar seasoned- teins were cloned, and the rules of expression of those genes has been studied greatly. Transgenic animals were utilized in learning the functionality of the gene in que- stion. In-situ hybridization is being utilized to localize the positioning of construction and examine the rules of professional- duction of peptides or proteins.

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Extra resources for Recombinant DNA Technologies in Neuroendocrinology

Sample text

Using in vitro DNase Ifootprinting assays, several G R binding sites were detected within the 5' flanking region of the human ~-ADH gene (one of the class I ADH genes). When DNA fragments containing these GR binding sites were linked to either homologous or heterologous promoters, they conferred glucocorticoid-induced expression of reporter genes as assessed by transfection assays (Winter et al. 1990). These results indicate that the human ~-ADH gene contains cis-acting elements functionally identical to the GRE.

A. Gustafsson Scheidereit C, Geisse S, Westphal HM, Beato M (1983) The glucocorticoid receptor binds to defined nucleotide sequences near the promoter of mouse mammary tumor virus. Nature 304:749-752 Schlechte JA, Ginsberg BH, Sherman BM (1982) Regulation of the glucocorticoid receptor in human lymphocytes. J Steroid Biochem 16:69-74 Schmid W, Striihle U, Schutz G, Schmitt J, Stunnenberg H (1989) Glucocorticoid receptor binds cooperatively to adjacent recognition sites. EMBO J 8:2257-2263 Schiile R, Miiller M, Otsuka-Murakami H, Renkawitz R (1988) Cooperativity of the glucocorticoid receptor and the CACCC-box binding factor.

J BioI Chem 260:3684-3690 Slater EP, Rabenau 0, Karin M, Baxter JD, Beato M (1985) Glucocorticoid receptor binding and activation of a heterologous promoter by dexamethasone by the first intron of the human growth hormone gene. Mol Cell BioI 5:2984-2992 Smith K, Shuster S (1984) Effect of adrenalectomy and steroid treatment on rat skin cytosol glucocorticoid receptor. J Endocrinol102:161-165 Stevens J, Stevens Y-W, Haubenstock H (1983) Molecular basis of glucocorticoid resistance in experimental and human leukemia.

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Recombinant DNA Technologies in Neuroendocrinology by D. Richter (auth.), Hiroo Imura M. D. (eds.)


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