Download PDF by Gary K. Ackers (auth.), Nicholas Catsimpoolas (eds.): Methods of Protein Separation
By Gary K. Ackers (auth.), Nicholas Catsimpoolas (eds.)
This open-end treatise on tools touching on seasoned tein separation had its starting in an American Chemical Society symposium entitled "Con transitority Protein Separation equipment" which used to be held in Atlantic urban, New Jersey in September 1974. the aim of the symposium-and subse quently of the current work-was to study the on hand glossy ideas and underlying ideas för attaining one of many extremely important initiatives of experimental biology, specifically the separation and characterization of proteins found in complicated organic combinations. Physicochemical characterization used to be lined simply as concerning the father or mother approach to fractionation and there fore concerned more often than not mass shipping strategies. also, the presentation of equipment for gaining perception into advanced interacting protein profiles used to be thought of of paramount value within the interpretation of separation styles. ultimately, particular different types of proteins (e. g. , chemically converted, deriving from a selected tissue, conjugated to varied moieties, and so on. ) require meticulous trial and choice and/or amendment of latest technique to hold out the specified separation. In such instances, the won event offers worthy instructions for additional experimentation. even if strong concepts exist at the present time for the separation and comparable physicochemical characterization of professional teins, many organic fractionation difficulties require extra ideas. it truly is was hoping that the outline within the current treatise of a few of the to be had separation instruments and their boundaries will give you the worthwhile built-in history for brand new advancements during this region. Nicholas Catsimpoolas Cambridge, Massachusetts vü CONTENTS Contents of quantity 1 . xvii bankruptcy 1 Scanning Gel Cbromatography Gary okay. Ackers I.
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Extra resources for Methods of Protein Separation
245:5166. Halvorson, H. , and Ackers, G. K. (1971). Axial dispersion of solute zones in gel permeation chromatography. J. Polymer Sei. Part A2 9:245. 26 GARY K. ACKERS Halvorson, H. , and Ackers, G. K. (1974). Molecuiar sieve studies ofinteracting protein systems. XI. Isomerizing solutes. J. Biol. Chern. 249:967. Henn, S. , and Ackers, G. K. (1969). Molecular sieve studies of interacting protein systems. V. Association of subunits of D-amino acid oxidase apoenzyme. Biochernistry 8:3829. Jones, M.
Eur. J. Biochem. 23:267. , and Mire, M. (1971b). Analytical-band centrifugation of an active enzyme-substrate complex. 2. Determination of active units of various enzymes. Eur. J. Biochem. 23:276. , and Messiah, A. (1967). Theory and practice of the analytical centrifugation of an active substrate--enzyme complex. Biopolymers 5:203. Fish, W. , Reynolds, J. , and Tanford, C. (1970). Gel chromatography of proteins in denaturing solvents: Comparison between sodium dodecyl sulfate and guanidine hydrochloride as denaturants.
CZE should also be used where ionic additions to a MZE system would seriously perturb its operative properties; this entails sacrificing the convenience of automatic, electrophoretic sampie concentration. For the purposes of this discussion, it is assumed, however, that the species of interest is available at a relatively low concentration, as is usually the case with components of biological systems. Under those circumstances, zone electrophoresis cannot be meaningfully carried out except under the restrictions of MZE.
Methods of Protein Separation by Gary K. Ackers (auth.), Nicholas Catsimpoolas (eds.)