Richard Pflanzer's Experimental and Applied Physiology Laboratory Manual PDF
By Richard Pflanzer
Experimental and utilized body structure Laboratory handbook, 8th version, is a complete, stand-alone laboratory handbook for the one-semester body structure path taught on the undergraduate point. it could possibly accompany any body structure textbook out there, and reinforces these rules which are primary to all classes on body structure. The strengths of this lab handbook are its emphasis on hands-on experiments, a realistic stability of history info, and transparent procedural directions.
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Additional resources for Experimental and Applied Physiology Laboratory Manual
Neural Control of Skeletal Muscle Achilles tendon as close as possible to its attachment at the heel. 6. Divide the tibia at the knee joint and cut away the remaining part of the lower leg. The preparation should now consist of the gastrocnemius muscle with severed Achilles tendon, the sciatic nerve attached to the muscle, and the knee joint with parts of the femur and tibia. 3. 7. Place the frog on the glass plate and cover it with a napkin soaked in frog Ringer’s solution. The remaining leg may be used by other students in the laboratory.
High-power objective = 45×, ocular = 10×; total magnification = (45×)(10×) = 450×. d. Oil-immersion objective = 100×, ocular = 10×; total magnification = (100×)(10×) = 1000×. 5. Arm: This curved portion of the microscope holds the body tube and stage. 2 © The McGraw−Hill Companies, 2003 Text 6. Stage: This platform supports the microscope slide while it is being viewed. A slide may be held in place for viewing by either a pair of stage clips or a mechanical stage clamp. 7. Diaphragm and condenser: Located below the stage on the substage, the diaphragm is used to adjust the amount of light passing through the slide into the lens system of the microscope, thus controlling the contrast.
2). 17. Revolve the turret until the low-power objective snaps into alignment with the body tube. 2 Field of view (FOV) as seen at three degrees of magnification containing the microfilm, and place the prepared slide of stained blood cells on the stage. Focus on the slide, using the low-power objective at first, and then using the high-power objective. Repeat steps 8–12, if necessary. Pflanzer: Experimental and Applied Physiology Laboratory Manual, Seventh Edition 3. Compound Light Microscopy 18.
Experimental and Applied Physiology Laboratory Manual by Richard Pflanzer