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Read e-book online Biochemical Regulation of Myocardium PDF

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By Matti Vornanen (auth.), Roland Vetter, Ernst-Georg Krause (eds.)

ISBN-10: 1461285518

ISBN-13: 9781461285519

ISBN-10: 1461312892

ISBN-13: 9781461312895

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References I. Gilman AG: G proteins: transducers of receptor-generated signals. Annu Rev Biochem 56; 615--649, 1987 2. McMahon KK: Developmental changes of the G proteins-muscarinic cholinergic receptor interactions in rat heart. J Pharmacol Exp Ther 251: 372-377,1989 3. Brown AM: Regulation of heartbeat by G protein-coupled ion channels. Am J Physiol259: HI621-HI628, 1990 4. Karczewski P, Bartel S, Krause EG: Differential sensitivity to isoprenaline oftroponin I and phospholamban phosphorylation in isolated rat hearts.

IOOOx, b. 400x, c. IOOOx). Fig. 5. Immuno-electron microscopy, preembedding method with HRP-DAB. (5A): a I subunit immunoreactivity can be found localized on sarcolemma and subsarcolemmal cisterns of adult rat myocytes.

The membranes were blocked with 3% ovalbumin in TBSbuffer and further proceeded as described by [32]. For the immunodetection of G proteins the followed dilutions were used: Gsa (l :250), Giac (1 : 150), Gia3 (1: 150), Goa (l :250), Gqa (l :200) and the antibody against the ~ subunit was diluted by 1 :250, respectively. Phospholamban was immunological detected in the crude membrane fraction. The proteins were separated by electrophoresis on 12% acrylamide gels. The transblots were reacted with a monoclonal antibody (1 :400 dilution).

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Biochemical Regulation of Myocardium by Matti Vornanen (auth.), Roland Vetter, Ernst-Georg Krause (eds.)

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